miniCGBE1-NG (pBM1182)

LOCUS       miniCGBE1-NG (pBM1182)                9195 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  CMV promoter expression plasmid for 
            rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    miniCGBE1-NG (pBM1182)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Kurt IC, Zhou R, Iyer S, Garcia SP, Miller BR, Langner LM, Grunewald
            J, Joung JK
  TITLE     CRISPR C-to-G base editors for inducing targeted DNA transversions 
            in human cells.
  JOURNAL   Nat Biotechnol. 2020 Jul 20. pii: 10.1038/s41587-020-0609-x. doi: 
            10.1038/s41587-020-0609-x.
  PUBMED    32690971
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             6..23
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(46..63)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    52..276
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(347..363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(347..363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(360..382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    371..387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(395..425)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    440..461
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(578..595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(749..1337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(829..848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1508..2368)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2131..2150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2369..2473)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2552..2571)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2743..3122
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3123..3326
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3277..3297
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     3368..3387
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3368..3386
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3436..3456
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4237..5952
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             8380..8400
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8419..8475
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8476..9192
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8521..8542)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8782..8801)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9129..9150
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
       61 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      121 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      181 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca
      241 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct
      301 ggggctcgat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
      361 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      421 gtaaagccta gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
      481 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
      541 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
      601 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
      661 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
      721 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
      781 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
      841 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
      901 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
      961 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1021 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1081 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1141 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     1201 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1261 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1321 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac actcagtgga
     1381 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1441 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     1501 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     1561 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     1621 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     1681 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     1741 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     1801 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     1861 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     1921 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     1981 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2041 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2101 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2161 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2221 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2281 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2341 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     2401 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     2461 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc
     2521 gatctcccga tcccctaggg tcgactctca gtacaatctg ctctgatgcc gcatagttaa
     2581 gccagtatct gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt
     2641 aagctacaac aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc
     2701 gttttgcgct gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta
     2761 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
     2821 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     2881 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     2941 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3001 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     3061 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     3121 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     3181 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     3241 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     3301 ggtgggaggt ctatataagc agagctggtt tagtgaaccg tcagatccgc tagagatccg
     3361 cggccgctaa tacgactcac tatagggaga gccgccacca tgaaacggac agccgacgga
     3421 agcgagttcg agtcaccaaa gaagaagcgg aaagtctcct cagagactgg gcctgtcgcc
     3481 gtcgatccaa ccctgcgccg ccggattgaa cctcacgagt ttgaagtgtt ctttgacccc
     3541 cgggagctgg ccaaggagac atgcctgctg tacgagatca actggggagg caggcactcc
     3601 atctggaggc acacctctca gaacacaaat aagcacgtgg aggtgaactt catcgagaag
     3661 tttaccacag agcggtactt ctgccccaat accagatgta gcatcacatg gtttctgagc
     3721 tggtcccctt gcggagagtg tagcagggcc atcaccgagt tcctgtccag atatccacac
     3781 gtgacactgt ttatctacat cgccaggctg tatcaccacg cagacccaag gaataggcag
     3841 ggcctgcgcg atctgatcag ctccggcgtg accatccaga tcatgacaga gcaggagtcc
     3901 ggctactgct ggcggaactt cgtgaattat tctcctagca acgaggccca ctggcctagg
     3961 tacccacacc tgtgggtgcg cctgtacgtg ctggagctgt attgcatcat cctgggcctg
     4021 cccccttgtc tgaatatcct gcggagaaag cagccccagc tgaccttctt tacaatcgcc
     4081 ctgcagtctt gtcactatca gaggctgcca ccccacatcc tgtgggccac aggcctgaag
     4141 tctggaggat ctagcggagg atcctctggc agcgagacac caggaacaag cgagtcagca
     4201 acaccagaga gcagtggcgg cagcagcggc ggcagcgaca agaagtacag catcggcctg
     4261 gccatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc
     4321 aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga
     4381 gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga
     4441 agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag
     4501 atggccaagg tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag
     4561 gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac
     4621 gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc
     4681 gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg
     4741 atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat ccagctggtg
     4801 cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt ggacgccaag
     4861 gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat cgcccagctg
     4921 cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct gggcctgacc
     4981 cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct gagcaaggac
     5041 acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta cgccgacctg
     5101 tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct gagagtgaac
     5161 accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga cgagcaccac
     5221 caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa gtacaaagag
     5281 attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg agccagccag
     5341 gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac cgaggaactg
     5401 ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga caacggcagc
     5461 atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca ggaagatttt
     5521 tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt ccgcatcccc
     5581 tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac cagaaagagc
     5641 gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc ttccgcccag
     5701 agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa ggtgctgccc
     5761 aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa agtgaaatac
     5821 gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa ggccatcgtg
     5881 gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga ggactacttc
     5941 aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg gttcaacgcc
     6001 tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt cctggacaat
     6061 gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt tgaggacaga
     6121 gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa agtgatgaag
     6181 cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct gatcaacggc
     6241 atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga cggcttcgcc
     6301 aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga ggacatccag
     6361 aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa tctggccggc
     6421 agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga gctcgtgaaa
     6481 gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga gaaccagacc
     6541 acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga gggcatcaaa
     6601 gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct gcagaacgag
     6661 aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca ggaactggac
     6721 atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt tctgaaggac
     6781 gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa gagcgacaac
     6841 gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct gctgaacgcc
     6901 aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg cggcctgagc
     6961 gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca gatcacaaag
     7021 cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa tgacaagctg
     7081 atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt ccggaaggat
     7141 ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga cgcctacctg
     7201 aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag cgagttcgtg
     7261 tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga gcaggaaatc
     7321 ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt caagaccgag
     7381 attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa cggcgaaacc
     7441 ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt gctgagcatg
     7501 ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag caaagagtct
     7561 atccggccca agaggaacag cgataagctg atcgccagaa agaaggactg ggaccctaag
     7621 aagtacggcg gcttcgtgag ccccaccgtg gcctattctg tgctggtggt ggccaaagtg
     7681 gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat caccatcatg
     7741 gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg ctacaaagaa
     7801 gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct ggaaaacggc
     7861 cggaagagaa tgctggcctc tgcccggttc ctgcagaagg gaaacgaact ggccctgccc
     7921 tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa gggctccccc
     7981 gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct ggacgagatc
     8041 atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa tctggacaaa
     8101 gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc cgagaatatc
     8161 atccacctgt ttaccctgac caatctggga gcccctcggg ccttcaagta ctttgacacc
     8221 accatcgacc ggaaggtgta ccggagcacc aaagaggtgc tggacgccac cctgatccac
     8281 cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg aggtgactct
     8341 ggcggctcaa aaagaaccgc cgacggcagc gaattcgagc ccaagaagaa gaggaaagtc
     8401 ggaggcggtg gaagcggcgc aacaaacttc tctctgctga aacaagccgg agatgtcgaa
     8461 gagaatcctg gaccgatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     8521 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     8581 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     8641 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     8701 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     8761 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     8821 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     8881 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     8941 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     9001 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     9061 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     9121 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     9181 gagctgtaca agtga
//